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NRP1對非小細胞肺癌放射敏感性的影響
來源: | 作者: | 發布時間:2014-4-15 17:32:03

NRP1對非小細胞肺癌放射敏感性的影響
董娟聰,張海芹,高輝,韓海玲,孟可,金順子
基金項目:國家高校博士點基金(No.2012006111) 作者簡介:董娟聰(1983-),女,博士研究生,主要從事放射醫學研究 通信聯系人:金順子(1965-),女,教授,主要從事輻射免疫學和輻射腫瘤學的研究.

(吉林大學公共衛生學院,衛生部放射生物重點實驗室)

摘要:目的:探討NRP1與不同種類非小細胞肺癌細胞株放射敏感性的關系,并篩選輻射抗性最強的細胞株,以便進一步研究NRP1在非小細胞肺癌產生輻射抗性中的作用機制。方法:通過Western blot 技術檢測不同來源的 5 種人非小細胞肺癌細胞株(H358、H460、H1299、A549、SK-MES-1) NRP1的基礎表達水平,使用深部X光機對5株細胞進行不同劑量照射,通過MTT實驗比較腫瘤細胞的輻射敏感性,初步篩選輻射敏感性最高與最低的 2 種細胞;10 進一步采用克隆形成實驗、劃痕實驗及流式細胞術分別檢測細胞存活分數、遷移能力及凋亡率的變化,分析 2 種細胞克隆形成能力、遷移及凋亡率與細胞放射敏感性的關系,并采用Western blot檢測照射后 2 種細胞 NRP1 的表達變化。結果:5 種腫瘤細胞NRP1表達量由高到低依次為:A549> SK-MES-1> H358> H460>H1299;X射線對腫瘤細胞的抑制能力隨著照射劑量的增加而增強,其抑制率由強到弱依次為:H460> H1299> H358> SK-MES-1> 15 A549,因此選取A549 細胞和 H460 細胞做后續研究?寺⌒纬蓪嶒烇@示,A549 在 2 Gy照射下的存活分數(SF2)是0.8872,而H460 細胞為0.3134。X 射線對A549 的遷移抑制力弱于 H460,10 Gy X射線作用后 H460 細胞的凋亡率明顯高于 A549 細胞,并且受照后 A549 細胞 NRP1 的表達量上調。結論:在非小細胞肺癌細胞中 A549 的輻射敏感性最弱,且輻射抗性的形成可能與NRP1表達上調有關。 20
關鍵詞: NRP1;非小細胞肺癌; A549;放射敏感性;輻射抗性
中圖分類號:R811.5
Efects of NRP1 on radiosensitivity of non small cell lung cancer 25
DONG Juancong, ZHANG Haiqin, GAO HUI, HAN Hailing, MENG KE, JIN Shunzi
(Key Laboratory of Radiobiology, Ministry of Health,Jilin University)
Abstract: Objective: To explore the relation of NRP1with the radiosensitivity of different types of non-small cell lung cancer. Methods: The expression of NRP1on the cells of H358、H460、H1299、A549 and SK-MES-1was detected by Western blot .MTT was used to detect cell proliferation, and 30 selected two cells which were the highest and lowest radiosensitivity ; The SF、Migration and apoptosis rate was detected with Clonogenic assay, wound healing assay and flow cytometry respectively. Results:The expression levels of NRP1 in the five cell lines (A549> SK-MES-1> H358> H460> H1299) were all increased by degrees in order.The radiosensitivity of five NSCLC cell lines were showed a dose-dependent decrease (A549Key words: Neuropilin 1; non-small cell lung cancer; A549; radiosensitivity; radioresistance

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