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基于連續切片的小鼠腎小管細段超微結構三維重建
來源: | 作者: | 發布時間:2013-11-12 15:28:06

基于連續切片的小鼠腎小管細段超微結構三維重建
溫昱,翟效月
(基礎醫學院,中國醫科大學,沈陽,110001)
摘要:目的:基于腎單位數字三維重建,闡明長髓袢腎單位細段超微結構和各型上皮分布范圍。方法:采用C57 小鼠腎組織連續半薄切片,數字三維重建,進行管狀追蹤,進行長髓袢腎單位細段不同類型上皮超微結構分析和分布。結果:長髓袢腎單位細段上皮分為2、3、4三型,其中2 型又分為2a、2b、2c 三個亞型。2 型上皮約占整個細段的(53.51±5.05)%,3 型占(38.47±3.06)%,余下的為4 型。2 型上皮中,2a 最為復雜彎曲,2b 和2c 上皮結構相對簡單。結論:長髓袢腎單位細段的超微結構和不同上皮分布規律,提示與其區域或節段性的離子運輸密切相關,是腎髓質滲透梯度形成的重要原因。
 關鍵詞:腎; 髓袢降支細段;長髓袢腎單位;上皮;小鼠
中圖分類號:R331

Ultrastructure of mouse thin limbs of Henle's loops based on erials sections and 3D reconstuction
WEN Yu, ZHAI Xiaoyue
(College of Basic Medical Sciences, C 5 hina Medical University, Shenyang, 110001)
Foundations: 高等學校博士學科點專項科研基金(20112104120014)
Brief author introduction:WEN Yu(1974), Female, Associate Professor,Histology.

Abstract: Thin limbs of the long looped nephrons (LLN) are of heterogenic ultrastructure and ransportation, which is important for formation of the gradient osmolarity in renal medulla and therefore the concentrated urine. Here presented a detailed description of the epithelia, including ultra-structure and length and location of the epithelial types and subtypes of the descending thin limbs(DTL), as well as ascending thin limbs (ATL), of the LLN. Three adult C57/BL/6J mice were used for
kidney tissue serial sections and computer assisted 3D reconstruction of nephrons. Based on tubular tracing, sections representing different epithelial types of DTL and ATL of the LLN were selected and re-embedded in Epon for the ultrastructural analysis. Three epithelial types, type-2, -3, and -4 in sequence are of their own ultrastructural features, running courses, and the spatial locations in renal medulla. Type-2 epithelium constituted the upper part of the DTL for a various distance, about
(53.51±5.05)%, located in the outer two thirds of the inner stripe of the outer medulla (ISOM); while type 3 epithelium lined the middle part (38.47±3.06)% of the DTL, started at the border of the outer two thirds and inner one third of the ISOM, ended at the lower 1/5 to 1/4 of the whole length of the DTL, at the various levels of the inner medulla (IM); and type-4 lined the last part of the DTL for various distances, and constituted the pre-bend part of the DTL and the whole length of the ATL. For the individual nephrons, the location of the transition between type-2 and -3 was almost consistent,while the transition of the type-3 and -4 was at the various levels of the IM. According to the ultrastructure, traditional type-2 epithelium was subdivided into type-2a, -2b, and -2c. Type-2a onstituted initial part of the DTL of LLN with highly complex ultrastructure and tortuous course for  various distances, whereas type-2b and -2c were structurally less complex with less tortuous course,
compared with type-2a, and gradually transferred into type-3 around the border of outer and inner medulla (check). In conclusion, different epithelial types and subtypes along DTL and ATL were arranged in a regular sequence, but of various lengths and locations, suggesting regional or segmental transportation properties.
Key words: Kidney; DTL; long-looped nephron; epithelium; mouse

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