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人工血管材料表面雙重功能化修飾促進血管原位內皮化
來源: | 作者: | 發布時間:2014-4-15 18:42:07

人工血管材料表面雙重功能化修飾促進血管原位內皮化
吉清,崔云,張素愛,王佳寧,王志紅,孔德領,趙強
基金項目:高等學校博士學科點專項科研基金(新教師類)(20100031120021);國家自然科學基金(青年基金)(81000680) 作者簡介:吉清,1988,女,碩士研究生,小口徑人工血管的表面修飾 通信聯系人:趙強,1978,男,副教授,生物活性材料.

(南開大學生物活性材料教育部重點實驗室,天津,300071)
摘要:在小口徑人工血管的研究中,血栓形成和內膜增生是導致血管狹窄的主要原因,因此防止血栓形成和促進材料表面快速內皮化是人工血管研究的一個重要方向。本文利用β-環糊精(β-CD)與金剛烷(AD)之間的主客體識別超分子組裝,對聚己內酯(PCL)材料表面進行了雙重功能化修飾。首先,制備了表面接枝β-CD的PCL膜,進而通過β-CD與AD之間主客體識別作用,將兩種不同功能分子修飾到PCL表面。其中一種是具有抗蛋白和血小板粘附的聚乙二醇(PEG),另一種是能夠特異性粘附內皮祖細胞(EPCs)的多肽TPSLEQRTVYAK (TPS)。進一步,系統評價了功能化表面的親疏水性和生物學性能,如纖維蛋白原粘附、血小板粘附和細胞的特異性捕捉等。實驗結果表明,PEG功能化修飾能顯著抑制血漿蛋白和血小板的粘附,從而降低了材料表面血栓形成的幾率。同時,TPS多肽的修飾則賦予材料表面特異性捕捉EPC的功能。本文所構建的雙重功能化修飾的材料表面作為人工血管植入后能夠促進內皮化進程,是一種具有良好應用前景的新型血管材料。
關鍵詞:功能高分子材料;表面功能化;主客體超分子組裝;內皮化;抗生物污染性能
中圖分類號:063-0

Surface functionalization of vascular grafts with the aim to promote in-situ endothelialization
Ji Qing, Cui Yun, Zhang Suai, Wang Jianing, Wang Zhihong, Kong Deling, Zhao Qiang
(Key Laboratory of Bioactive Materials,College of Life Science, Nankai University, 25 Tianjin,300071)
Abstract: In the case of small-diameter vascular grafts, the thrombosis and intimal hyperplasia is the main reason for occlusion after implantation. Hence, to develop materials with surface funtions to resist the thrombus formation and promote endothelialization is an important aspect in the research of vascular grafts.In this study, we developed a method for the dual functionalization of 30 poly(ε-caprolactone) (PCL) surface by the means of supramolecular assembly based on the host-guest inclusion complexation between β-cyclodextrin (β-CD) and admantane (AD). Firstyly, β-CD was grafted onto the PCL surface. Then two types of functional molecules were immobilized on the PCL surface through host-guest inclusion complexation between β-cyclodextrin (β-CD) grafted on the PCL surface and admantane (AD) motif at the chain end of 35 functional molecules. One functional molecule is polyethylene glycol (PEG), which is resistance to plasma protein and platelet adsorption, and the other is TPSLEQRTVYAK (TPS) peptide, which can specifically bind endothelial progenitor cells (EPCs).Finally, the hydrophilic/hydrophobic property of the functionalized surface as well as the fibrinogen adsorption, platelet adhesion, and specific capture of cells were systematically evaluated on the 40 functionalized surface. The results show that the presence of PEG has evidently inhibited adsorption of plasma protein and platelet adhesion, thus reducing the possibility of thrombus formation. At the same time, TPS-functionalized surface demonstrated selective adhesion towards EPC instead of other types of cells.All these results indicate that the dually functionalized surface developed in this study is a suitable candidate for vascular graft to induce or promote in-situ 45

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